Serotyping of Chlamydia: isolates of bovine origin
نویسندگان
چکیده
منابع مشابه
Serotyping of Chlamydia. I. Isolates of ovine origin.
Eight chlamydial isolates of ovine origin were tested in a plaque reduction system using homologous and heterologous rooster antisera. The eight isolates could be separated into two separate immunotypes. Type 1 included isolates associated with ovine abortion and one agent recovered from the feces of an apparently normal sheep. Type 2 isolates were associated with polyarthritis and conjunctivit...
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A panel of five serovar-specific monoclonal antibodies which distinguish the five known avian serovars of Chlamydia psittaci was used to serotype 45 European avian Chlamydia psittaci isolates. Chlamydial antigen was grown in Buffalo green monkey (BGM) cells or in embryonated chicken eggs and was then inoculated into BGM cells. Serotyping was performed in an indirect immunofluorescence test. The...
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A panel of 10 serovar-specific monoclonal antibodies that could distinguish 10 distinct serovars of Chlamydia psittaci was prepared. The panel included one monoclonal antibody to each of the 10 serovars. Monoclonal antibodies were selected for their specificity in the indirect microimmunofluorescence test. Each of the monoclonal antibodies had a titer of 1:1,280 or higher to the homologous stra...
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The protein analysis of Pasteurella multocida organism was performed by sodium dodycyle sulphate polyacrylamide gel electrophoresis (SDS-PAGE) technique using a high pH discontinous buffer system. Six isolates of the Pasteurella multocida organism were confirmed by Gram’s staining and different biochemical tests. In the protein fractions of purified Pasteurella multocida strains, seven polypept...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1975
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.11.5.904-907.1975